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Last Updated: Jul 15, 2026

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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Iron deficiency anemia, or IDA, is associated with an increased TIBC as there is less iron to bind to transferrin. Microcytic, hypochromic red cell morphology, a decreased serum iron level, a decreased serum ferritin level, and a decreased hemoglobin level are all characteristics associated with IDA.
All of the following are characteristic findings in patients with iron deficiency anemia EXCEPT:

A) decreased hemoglobin
B) microcytic, hypochromic red cell morphology
C) decreased ferritin
D) decreased total iron-binding capacity (TIBC)
E) decreased serum iron level


2. An antithetical relationship exists between the antigens M and N. Antithetical relationships occur in situations where for a given locus, only one of two genes may be inherited, the alleles are termed antithetical alleles.
In immunohematology, an antithetical relationship exists between M antigen and which of these antigens?

A) K
B) N
C) Vw
D) S


3. On the red cell membrane, there is a bicarbonate / chloride exchanger. This exchanger allows for bicarbonate to leave the red cells while chloride is allowed inside.
In blood, bicarbonate leaves the red blood cell and enters the plasma through an exchange mechanism with:

A) chloride
B) sodium
C) phosphorus
D) potassium


4. Blood bank
Once the seal on a unit of packed red cells is broken, how long can the unit be stored refrigerated prior to administration:

A) 24 hours
B) 48 hours
C) 4 hours
D) 12 hours


5. Hemoglobin electrophoresis uses an electric field to separate hemoglobin molecules based on differences in net electrical charge. The rate of electrophoretic migration is also dependent on the ionic radius of the molecule, the viscosity of the solution through which it is migrating, the electrical field strength, temperature, and the type of supporting medium used.
Electrophoretic separation of hemoglobin fundamentally relies on:

A) Size differences of molecules
B) Shape variations of molecules
C) Concentration differences of molecules
D) Net charge differences of molecules


Solutions:

Question # 1
Answer: D
Question # 2
Answer: B
Question # 3
Answer: A
Question # 4
Answer: A
Question # 5
Answer: D

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